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The original B7/21 hybridoma was derived from hybridization of mouse 5194/SXXOBU1 myeloma cells with spleen cells from BALB/c mice immunized with NALM6 human leukemia cells and secreted IgG3, κ antibody. An immunoglobulin switch-variant B7/21 hybridoma, also known as B7/21.49 or B7/21 (IgG1), was subsequently isolated that secreted lgG1 antibody with the same specificity as the original IgG3.
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B7-H5 expression and CD8 + T infiltration in CRC tissues. Next, we examined the relationship between B7-H5 expression and CD8 + T-cell infiltration in tissue samples of patients with CRC. As shown.
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B7-H5 inhibition suppressed tumor growth in mice and promoted CD8 + T-cell infiltration in the tumor tissues of MC38 model.. (B7-H5) antibody (CST, Danvers, MA, USA) and rabbit anti-human CD8α antibody (Abcam, Cambridge, MA, USA) at 4 °C overnight, and this was followed by incubation with the corresponding HRP-labeled secondary antibody.
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The end-result of stimulation of T-cell receptors by antigen is in part determined by co-signalling pathways such as the B7/CD28 axis. Zhu et al.identify a novel costimulatory CD28-like receptor.
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Description: This 7-517 monoclonal antibody reacts with human CD276, which is also known as B7-H3. This type I transmembrane protein is expressed on immature and mature dendritic cells but not monocytes, granulocytes, or resting lymphocytes. Moreover, CD276 is expressed on non-hematopoietic cells such as osteoblasts, fibroblasts, and epithelial.
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B7-H4 is reportedly overexpressed in a variety tumors. B7-H4 expression by tumor macrophages appears to play a role in suppression antigen-specific T cell mediated immunity. Recent studies indicate that B7-H4 can also be a positive regulator of T cell responses. B7-H4 can be involved innate immunity as well, eg, by inhibiting neutrophil.
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CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process.
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After blocking with 5% BSA in PBS, cell surface markers such as B7-H3, CD45, CD11B, CD3, CD4, CD8, F4_80, and CD86 were stained first with antibodies diluted at 1:200 for 40 min on ice, accompanied by Live/Dead staining using Fixable Viability Stain 700 (BD Biosciences) for 15 min. Stained cells were fixed with 4% paraformaldehyde (PFA) for 15.
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The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis. For Research Use Only. Not for use in diagnostic procedures.
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Introduction. Trastuzumab is a humanized monoclonal antibody (mAb) used to treat HER2-overexpressing breast cancer .Despite years of successful clinical use, the mechanisms of trastuzumab action are still being investigated .Although suppression of HER2 signaling was a primary focus of early mechanistic studies , subsequent studies have focused on the role of immunity in mediating trastuzumab.