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Sec62 consists of an N-terminal domain (NTD), two TMDs (TM1 and TM2) linked by a short ER luminal loop (L1/2), and a cytosolic C-terminal region [8], [11].. (PDB ID: 7KAI), whereas the C2 conformation reflects an intermediate degree of opening (PDB ID: 7KAJ). However, the cryo-EM structures can only partially reveal how the conformation and.
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Without Sec62, the translocation pore of Sec61 remains closed by the plug domain, rendering the channel inactive. We further show that the lateral gate of Sec61 must first be partially opened by interactions between Sec61 and Sec63 in cytosolic and luminal domains, a simultaneous disruption of which completely closes the channel.
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